|
KMID : 0895820130230040023
|
|
Journal of Oriental Rehabilitation Medicine
2013 Volume.23 No. 4 p.23 ~ p.37
|
|
|
Effects of Saengkanggamchotang (SKT) on MIA-Induced Osteoarthritis in Rats
|
|
Choi Bo-Mi
Oh Min-Seok
|
|
|
Abstract
|
|
|
Objectives : This study was performed to investigate the effects of Saengkanggamchot-ang(SKT) on the monosodium iodoacetate (MIA) induced osteoarthritis in rats.
Methods : Osteoarthritis was induced by injection of MIA (50 ul, 60 mg/ml) into knee joints of rats. Rats are divided into a total of 4 groups (normal, control, positive comparison group, SKT treated group, each n=6). Normal group are not treated at all without inducing osteoarthritis whereas control group were induced for osteoarthritis by MIA and oral medi-cated with 20 ml of distilled water per day. Positive comparison group was injected with MIA and after 7 days, that was taken indomethacin (30 mg/kg/mouse). SKT treated group was injected with MIA and after 7 days that was taken SKT (30 mg/kg/mouse). Positive comparison group and SKT treated group were oral medicated for each substance a total of 4 weeks with one time per day. After experiments (from 1 week after injection of papain to 4 weeks elapsed), the functions of liver and kidney, Prostaglandin E2, inflammatory cy-tokine (IL-1β, IL-6, TNF-α), osteocalcin, TIMP-1, MMP-9 within serum. Knee joint struc-tures were observed by H&E, safranin-O staining method, and amount of cartilage were measured by μCT-arthrography.
Results : 1) Hind paw weight bearing ability was significantly improved. 2) Functions of liv-er and kidney were not affected. 3) Prostaglandin E2, osteocalcin, TIMP-1, MMP-9 in se-rum were significantly decreased. 4) Inflammatory cytokine IL-1β was significantly de-creased, and IL-6, TNF-α were decreased but had not significant. 5) In terms of histo-pathology, significantly reduced subsidence of cartilage and bone in H&E staining. And in Safranin O staining, proteoglycan content in synovial membrane was significantly in-creased compared with control group. 6) Destruction of cartilage on μCT-arthrography was significantly reduced.
Conclusions : Based on all results mentioned above, Saengkanggamchotang (SKT) is believed to be meaningful for suppressing the progress of osteoarthritis and its treatments.
|
|
|
KEYWORD
|
|
|
Saengkanggamchotang, SKT, Osteoarthritis, Anti-inflammation, Monoindo-sodium iodoacetate, MIA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|